Cardiac autonomic nervous activity during different sleep stages in individuals with spinal cord injury: The influence of physical training.

OBJECTIVE: The present study assessed the influence of physical training on cardiac autonomic activity in individuals with spinal cord injury (SCI) during different sleep stages. METHODS: Twenty-six volunteers were allocated into three groups: 9 sedentary individuals without SCI (control, CON); 8 sedentary tetraplegic individuals with chronic SCI (SED-SCI); 9 physically trained tetraplegic individuals with chronic SCI (TR-SCI). All participants underwent nocturnal polysomnography to monitor sleep stages: wakefulness, non-rapid eye movement (NREM) sleep (N1, N2, and N3 stages), and REM sleep. The electrocardiography data obtained during this exam were extracted to analyze the heart rate variability (HRV). RESULTS: Sleep stages influenced HRV in the time [RR interval and root mean square of successive RR interval differences (RMSSD)] and frequency [low-frequency (LF) and high-frequency (HF) powers and LF-to-HF ratio] domains (P < 0.05). SED-SCI individuals showed unchanged HRV compared to CON (P > 0.05). When comparing the TR-SCI and SED-SCI groups, no significant differences in HRV were reported in the time domain (P > 0.05). However, in the frequency domain, more accentuated HF power was observed in TR-SCI than in SED-SCI individuals during the N2 and N3 stages and REM sleep (P < 0.05). Moreover, TR-SCI had higher HF power than CON during the N3 stage (P < 0.05). CONCLUSIONS: TR-SCI individuals have greater HF power, indicative of parasympathetic modulation, than sedentary (injured or not injured) individuals during different sleep stages. Therefore, enhanced parasympathetic activity induced by physical training may improve cardiac autonomic modulation during sleep in individuals with chronic SCI.

Sleep as a prognostic factor in low back pain: a systematic review of prospective cohort studies and secondary analyses of randomized controlled trials.

Sleep problems are common in individuals with low back pain (LBP) and sleep restriction seems to be associated with impaired pain processing. Our objective was to investigate whether sleep is associated with future LBP outcomes (i.e., pain intensity, disability, and recovery) in adults. We conducted a systematic review of prospective cohort studies and secondary analyses of randomized controlled trials (registration - PROSPERO CRD42022370781). In December 2022, we searched the MEDLINE, Embase, CINAHL, and PsycINFO databases. Fourteen studies, totaling 19,170 participants were included. Thirteen studies were rated as having high risk of bias (QUIPS tool). We used vote-counting and meta-analysis approaches to synthesize the data. We found associations between baseline sleep with future pain intensity, recovery, and between changes in sleep with changes in pain intensity, changes in disability, and recovery. We further synthesized outcomes as 'overall LBP improvement' outcome. Baseline poor sleep was moderately associated with non-improvement in LBP in the long-very long term (OR 1.55, 95% CI 1.39 to 1.73; three studies providing unadjusted effect sizes), and non-improvement in sleep was largely associated with non-improvement in LBP in the short-moderate term (OR 3.45, 95% CI 2.54 to 4.69; four studies providing unadjusted effect sizes). We found no association between baseline sleep with future disability and overall LBP improvement in the short-moderate term. Therefore, sleep may be a prognostic factor for pain intensity and recovery from LBP. All findings were supported by low to very low-quality evidence. Better-conducted studies are needed to strengthen our certainty about the evidence.

Performance, body development and ingestive behavior of Nelore and crossbred heifers finished in a grazing system.

The aim of this experiment was to evaluate growth, body development and ingestive behavior of Nelore and crossbred heifers. Twenty-two contemporary heifers (eight Nelore, seven Nelore × Angus (½ Angus) and seven Nelore × Pantaneiro (½ Pantaneiro) crosses) were evaluated. The variables evaluated were weight, subcutaneous fat thickness [assessed by ultrasound in the longissimus dorsi (SFT) and biceps femoris (SFTP8)] morphometric measures and ingestive behavior (determined between 7 am and 5 pm). The daily gain differed between the breeds (P < 0.05), being greater for the ½ Pantaneiro than Nelore and equal to ½ Angus at the end of the study. The ½ Angus heifers differed (P < 0.01) from the other breeds for SFT (4.36 mm), with no difference between Nelore (2.77 mm) and ½ Pantaneiro (3.38 mm). The SFTP8 was greater (P < 0.01) (5.36 mm) in ½ Angus heifers than t others crossbreed, and greater in ½ Pantaneiro than in Nelore (4.28 vs 3.29 mm). The heart girth and rump width between the ilia were larger (P < 0.01) for the ½ Angus than ½ Pantaneiro and Nelore (169 cm; 41.6 cm, respectively). Substernal height was greatest (P < 0.01) for the Nelore (69.1 cm), reflecting the greater anterior and posterior height. The ½ Angus spent the longest time (P < 0.05) walking and less time grazing. Idleness also differed (P < 0.05) throughout the day. Crossbred heifers exhibited better performance and development than Nelore. The grazing pattern of ½ Pantaneiro heifers was identical to Nelore, but walked less, gained more weight, and exhibited better carcass finishing.

Perillyl Alcohol Promotes Relaxation in Human Umbilical Artery.

BACKGROUND: Perillyl alcohol (POH) is a monoterpenoid found in plant essential oils and has been shown to relax murine vessels, but its effect on human vessels remains poorly studied. OBJECTIVE: The study aimed to characterize the effect of POH on human umbilical arteries (HUA). METHODS: Rings of HUA were obtained from uncomplicated patients and suspended in an organ bath for isometric recording. The vasorelaxant effect of POH in HUA was evaluated on basal tone and electromechanical or pharmacomechanical contractions, and possible mechanisms of action were also investigated. RESULTS: POH (1-1000 µM) altered the basal tone of HUA and completely relaxed HUA rings precontracted with KCl (60 mM) or 5-HT (10 µM), obtaining greater potency in the pharmacomechanical pathway (EC50 110.1 µM), suggesting a complex interference in the mobilization of extra- and intracellular Ca2+. POH (1000 µM) inhibited contractions induced by BaCl2 (0.1-30 mM) in a similar way to nifedipine (10 µM), indicating a possible blockade of L-type VOCC. In the presence of potassium channel blockers, tetraethylammonium (1 mM), 4-aminopyridine (1 mM), or glibenclamide (10 µM), an increase in the EC50 value of the POH was observed, suggesting a modulation of the activity of BKCa, KV, and KATP channels. CONCLUSION: The data from this study suggest that POH modulates Ca2+ and K+ ion channels to induce a relaxant response in HUA.

PI3K Signaling Pathways as a Molecular Target for Glioblastoma Multiforme.

Glioblastoma multiforme (GBM) is the most common type of cancer that affects the central nervous system (CNS). It currently accounts for about 2% of diagnosed malignant tumors worldwide, with 296,000 new cases reported per year. The first-choice treatment consists of surgical resection, radiotherapy, and adjuvant chemotherapy, which increases patients' survival by 15 months. New clinical and pre-clinical research aims to improve this prognosis by proposing the search for new drugs that effectively eliminate cancer cells, circumventing problems such as resistance to treatment. One of the promising therapeutic strategies in the treatment of GBM is the inhibition of the phosphatidylinositol 3-kinase (PI3K) pathway, which is closely related to the process of tumor carcinogenesis. This review sought to address the main scientific studies of synthetic or natural drug prototypes that target specific therapy co-directed via the PI3K pathway, against human glioblastoma.

Prevalence and incidence of health problems and their characteristics in Brazilian para athletes: A one-season single-center prospective pilot study.

BACKGROUND: Para athletes experience high prevalence and incidence of health problems related to sport. Despite this, there are few longitudinal studies. OBJECTIVE: To describe the characteristics, prevalence, incidence, and severity of health problems in para athletes from one of the Brazilian Paralympic Reference Centers during a sports season and to compare the prevalence of health problems between para athletics, para powerlifting, and para swimming. METHODS: This prospective pilot study was conducted from October 2019 to March 2020. The Oslo Sports Trauma Research Center Questionnaire on Health Problems was used to record injuries and illnesses every week for 24 weeks. The characteristics, prevalence, incidence, and severity of health problems were described for each modality. The prevalence of health problems was compared among the three sport modalities. RESULTS: Thirty-five para athletes participated. Most of the injuries occurred in the shoulder, and most illnesses caused respiratory and gastrointestinal symptoms. The average weekly prevalence and the incidence rate of health problems were 40.6% (95% CI 17.0-64.4) and 12.7 (95% CI 9.6-15.9) per 1000 athlete hours, respectively. Para powerlifting had the highest prevalence of all and substantial health problems; para swimming had the lowest prevalence of injuries; and para athletics had the lowest prevalence of illnesses. CONCLUSIONS: This group of Brazilian para athletes showed a high prevalence and incidence of health problems throughout the season. Para athletics, para powerlifting, and para swimming each had a different prevalence of injuries and illnesses.

Especialização em Educação Popular em Saúde na Promoção de Territórios Saudáveis e Sustentáveis da Fiocruz: uma análise sob a ótica dos próprios educandos / Specialization in Popular Health Education in the Promotion of Healthy and Sustainable Territories at Fiocruz: an analysis from the students' perspective

RESUMO Este estudo objetivou compreender a experiência do curso de Especialização em Educação Popular em Saúde na Promoção de Territórios Saudáveis e Sustentáveis da Fundação Oswaldo Cruz (Fiocruz), sob a ótica dos educandos. Foi realizada uma pesquisa qualitativa por meio de entrevistas semiestrutu-radas feitas com estudantes do curso (n = 20). Os dados foram interpretados pela análise de conteúdo temática de Bardin. Os resultados compuseram três categorias temáticas: a Pedagogia da Formação, que apresenta a compreensão das estratégias formativas e metodológicas do curso; a Pedagogia do Encontro, que traz as potências que se fizeram no encontro entre as diversidades; a Pedagogia do Movimento, que levanta as implicações do curso para a transformação pessoal e profissional dos participantes. A formação apresentou-se como um potente instrumento de transformação de perspectivas e de práticas, em que o encontro atuou como via de articulação política e de cuidado à saúde.

ABSTRACT This study aimed to understand the experience of the Specialization course in Popular Health Education in the Promotion of Healthy and Sustainable Territories at Oswaldo Cruz Foundation (Fiocruz), from the student's perspective. A qualitative research was carried out through semi-structured interviews with students from the course (n = 20). Data were interpreted by Bardin's thematic content analysis. The results comprised three thematic categories: the Pedagogy of Training, which presents the understanding of the training and methodological strategies of the course; the Pedagogy of the Encounter, which brings the powers that have been made in the encounter between diversity; the Pedagogy of Movement, which raises the implications of the course for the personal and professional transformation of the participants. Training presented itself as a powerful instrument for transforming perspectives and practices, in which the meeting acted as a means of political articulation and health care.

The Sleep Parameters of Olympic Athletes: Characteristics and Assessment Instruments.

This systematic review aims to identify the sleep parameters of Olympic athletes and the instruments used to assess and monitor the sleep of these athletes. The search was conducted until February 2023 and was performed in PubMed, Web of Science, and Scopus databases. This systematic review has included studies that investigated at least one of the following sleep parameters: total sleep time (TST), sleep onset latency (SOL), sleep efficiency (SE), awakenings after sleep onset (WASO), quality of sleep, daytime sleepiness, and chronotype; the participants were Olympic athletes. The search returned a total of 280 studies. After screening based on exclusion and inclusion criteria, 11 studies were included. The main results demonstrate that Olympic athletes have TST of 06:10 h, SE of 84%, SOL of 28 min, and WASO of 49 min. The most predominant chronotype is indifferent; over half of the athletes have poor sleep quality and complaints. Furthermore, actigraphy was the most used method to assess sleep. It is concluded that Olympic athletes have TST, SE, and WASO poor than the recommended values. In addition, sleep complaints and poor sleep quality were also observed. Among the objective sleep assessment methods, actigraphy was the method most frequently used in this population.

Cognitive dysfunction in systemic lupus erythematosus is associated with disease activity and oxidative stress: a comparative study with rheumatoid arthritis for identifying biomarkers.

BACKGROUND: The prevalence and pathophysiological mechanisms of cognitive deficits (CD) Systemic Lupus Erythematosus (SLE) and Rheumatoid arthritis (RA) are very heterogeneous and poorly understood. We characterized CD in patients with SLE compared with RA patients and healthy controls. We compared the neuropsychological profile of SLE and RA with patients' oxidative/inflammatory biomarkers for CD. METHODS: We performed a cross-sectional study, including 50 SLE patients, 29 RA patients, and 32 healthy controls. SLEDAI and DAS28 assessed disease activity. SF-36 questionnaire and a battery of cognitive tests were applied to all participants. Blood samples were collected to determine IL-6, S100ß, myeloperoxidase (MPO), malondialdehyde and reduced glutathione (GSH) alterations. RESULTS: In the SLE group, higher GSH was associated with the absence of CD (With CD = 69 ± 49, Without CD = 112 ± 81, p = 0.030), while higher IL-6 was associated with the presence of CD in the RA group (With CD = 603 ± 173, Without CD = 431 ± 162, p = 0.032). Regarding specific cognitive domains, in SLE higher MPO was associated with poor performance in reasoning and abstraction (p = 0.039), higher IL-6 was associated with poor performance in inhibitory control and attention (p = 0.031), and higher GSH was associated with better performance in memory(p = 0.021). Higher SLEDAI was associated with poor performance in semantic fluency(p = 0.031), inhibitory control, and attention in the SLE group(p = 0.037). In the RA group, higher DAS-28 was associated with poor performance in executive functions(p = 0.016) and phonemic fluency (p = 0.003). CONCLUSION: SLE patients' disease activity, inflammatory state, and oxidative stress were associated with CD. In RA patients, CD was associated with disease activity and inflammatory state. These results encourage further studies with larger samples aiming to confirm oxidative stress parameters as biomarkers of CD in SLE patients.

The Effectiveness of Topical Dimethicone Together with a One Health Approach for the Control of Tungiasis in the Sanumás Communities, Yanomami Territory, Amazon Rainforest: A Real-World Study.

BACKGROUND: The success of tungiasis treatment is highly dependent on adequate environmental control. METHODS: This is a real-world observational cohort study designed to monitor the effectiveness of topical dimethicone together with a One Health approach for the control of tungiasis in the Sanumás communities, Amazon rainforest, Brazil. We followed up on 562 indigenous people and 81 domestic dogs for 1.5 years in a 3-month interval. A new molecular method for large-scale soil evaluation was also tested. The control of tungiasis was independently conducted by the Brazilian Ministry of Health and comprised topical dimethicone application (NYDA®) for humans, single-dose oral afoxolaner for dogs, and in-house soil fumigation with fipronil. The main outcome was the occurrence of tungiasis after the use of topical dimethicone together with the One Health approach. RESULTS: A total of 49 of the 562 indigenous people had active tungiasis at enrollment (8.72%). Only three cases of tungiasis resulted in active lesions after the use of topical dimethicone together with the One Health approach, with two cases of recurrence. From the 6-month follow-up and after, soil infestation was not detected. CONCLUSIONS: We conclude that the use of NYDA® together with animal and environmental interventions are effective measures for the control of tungiasis.

Efeitos de uma Intervenção Psicológica no Estresse, Ansiedade, Concentração e Desempenho Físico em Bailarinas / Effects of Psychological Intervention on Stress, Anxiety, Concentration and Physical Performance in Dancers

Bailarinos sofrem pressões por bons desempenhos, tendo em vista que ao nível profissional sua prática é performática e exige resultados. Assim, avaliar e intervir com essa população é necessário. Objetivou-se avaliar os possíveis efeitos de uma intervenção psicológica nos níveis de estresse, ansiedade, concentração e desempenho físico de bailarinas adultas. Participaram do estudo onze bailarinas com idade entre 18 e 23 anos, que competiam nacional e internacionalmente na modalidade Jazz. Foram realizadas 14 sessões com duração média de 50 minutos cada, uma vez por semana. Trabalhou-se com psicoeducação, estabelecimento de metas, controle de estresse, ansiedade, atenção e concentração, entre outros. Por meio de testes específicos (Escala de Percepção de Estresse, Teste de Ansiedade de Competição Esportiva, Teste de Atenção Seletiva e desempenho físico através de banca julgadora), aplicados antes e após a intervenção, percebeu-se melhora da ansiedade e concentração, assim como de aspectos do desempenho físico, como qualidade técnica, tempo musical e performance geral. Ressalta-se a importância do acompanhamento psicológico, desde a avaliação até a intervenção, não apenas para melhorar o desempenho, mas também a saúde e qualidade de vida desta população.

Dancers are pressured by good performances, considering that at a professional level their practice is performative and requires results. Thus, evaluating and intervening with this population is necessary. The aim was to evaluate the possible effects of a psychological intervention on the levels of stress, anxiety, concentration and physical performance of adult dancers. Eleven dancers, aged between 18 and 23 years, who competed nationally and internationally in the Jazz modality, took part in the study. There were 14 weekly sessions with an average duration of 50 minutes each. We worked with psychoeducation, goal setting, stress control, anxiety, attention and concentration, among others. Through the specific tests (Stress Perception Scale, Sports Competition Anxiety Test, Selective Attention Test and physical performance through a judging panel), applied before and after the intervention, an improvement in anxiety and concentration was noticed, through the previously mentioned tests, as well as in aspects of physical performance, such as technical quality, musical time and general performance. The importance of psychological monitoring is emphasized, from evaluation to intervention, not only to improve performance, but also the health and quality of life of this population.

Can the inner eye canthus temperature be used as an alternative method to measure core temperature in sleep-deprived individuals?

Core temperature is used in several situations, including studies on biological rhythms and circadian markers of physical performance. Measuring the inner eye canthus (Tco) temperature is a method proposed to identify core temperature, but it has shown little concordance in physical exercise situations and has not yet been used in studies with measurements taken throughout the day. The objective of this study was to compare the measurements and daily behavior of Tco obtained by infrared thermography with rectal temperature (Tre) during a prolonged waking protocol. Eleven male individuals participated in the study, who remained in the laboratory for at least 38 h using an actigraph to determine the wakefulness time and were monitored during the entire period. The Tre and Tco measurements were performed every 3 h. The ANOVA was used for repeated measurements followed by Bonferroni's post-hoc test to find the limits of concordance/proximity, while the Bland and Altman method and the Intraclass Correlation Coefficient were used to establish the reliability between the pairs. The significance level adopted was p < 0.05. The results demonstrate significant differences, low levels of concordance and unsatisfactory reliability levels between Tco and Tre at all 13 analyzed moments, in addition to not showing measurement reliability when all data are used together with the 143 temperature measurements. Daily behavior analysis shows moments with similar behavior with an increase in Tco and Tre, but at other times the behavior was the opposite, with a decrease in one measurement and an increase in the other. Based on the results presented, it is not recommended to use the inner eye canthus temperature as a substitute for rectal temperature for measuring core temperature at different times of the day or in sleep-deprived individuals.

Vasorelaxant effect of (E,E)-farnesol in human umbilical vein ex vivo assays.

(E,E)-farnesol is a sesquiterpene acyclic alcohol produced by bacteria, protozoa, fungi, plants, and animals. The literature describes its applications in food, pharmaceutical, and cosmetic industries, and also in the pharmacological context with a vasorelaxant effect. However, its effects on human umbilical vessels remain poorly investigated. Thus, this study aims to investigate, in a new way, the vasorelaxant effect of (E,E)-farnesol in human umbilical veins (HUV) from healthy donors. Rings obtained from isolated HUV were suspended in an organ bath to record their isometric tension in different experimental sections. (E,E)-farnesol (1 µmol/L to 1 mmol/L) promoted vasorelaxant effect in venous preparations contracted by depolarization (KCl 60 mmol/L) or pharmacological agonism (5-HT 10 µmol/L), with EC50 values of 239.9 µmol/L and 424 µmol/L, respectively. In calcium-free solution, this effect was also observable. (E,E)-farnesol was able to suppress contractions evoked by CaCl2 and BaCl2 suggesting a blockade of voltage-dependent (especially L-type) calcium channels. The vasorelaxant efficacy and potency of (E,E)-farnesol were affected in the presence of tetraethylammonium (1 and 10 mmol/L), glibenclamide (10 µmol/L) and BaCl2 (1 mmol/L) indicating a possible involvement of potassium channels (BKCa, KATP and KIR) in this effect. Our data suggest that (E,E)-farnesol has a promising potential to be applicable as a vasodilator in hypertensive conditions in pregnancy that alter HUV reactivity.

Comentários nas mídias sociais e a banalização da violência psicológica contra as mulheres / Comments on social media and the trivialization of psychological violence against women / Comentarios en las redes sociales y la banalización de la violencia psicológica contra las mujeres

A violência psicológica é frequente, porém, de difícil detecção, muitas vezes presente em relacionamentos abusivos e confundida com ciúmes e cuidado. Nas mídias sociais isso pode ser incentivado através de postagens, assim como pode servir de espaço de suporte e compartilhamento de experiências. Objetivou-se analisar comentários publicados em mídias sociais relacionados à violência contra a mulher e sua associação à violência psicológica. Utilizou--se o método da netnografia, através de análise textual dos comentários de um vídeo publicado na rede social Facebook chamado: "Não confunda amor com abuso". Foram transcritos 571 comentários da publicação, sendo o corpus analisado pelo software Iramuteq. Os comentários analisados mostram que questões culturais contribuem para a banalização da violência contra a mulher, principalmente da violência psicológica, pois geralmente só é considerado como violência quando há agressão física. Percebe-se a importância de um trabalho de psicoeducação, como por exemplo através de vídeos orientativos, visando a conscientização da população e possível redução da violência. (AU)

Psychological violence is frequently encountered but often difficult to detect, as it is commonly present in abusive relationships and can be mistaken for jealousy and care. In the realm of social media, it can either be encouraged through posts or serve as a space for support and the sharing of experiences. The objective of this study was to analyze comments posted on social media platforms related to violence against women and its association with psychological violence. The netnography method was employed, involving textual analysis of comments on a video posted on the social media platform Facebook, titled "Não confunda amor com abuso" (Do Not Confuse Love with Abuse). A total of 571 comments from the post were transcribed and analyzed using the Iramuteq software. The analyzed comments reveal that cultural factors contribute to the trivialization of violence against women, particularly psychological violence, since the term violence is often only recognized when it escalates to physical aggression. The significance of psychoeducational efforts, such as informative videos, becomes apparent in raising awareness among the population and potentially reducing instances of violence. (AU)

La violencia psicológica es frecuente, sin embargo, difícil de detectar, a menudo presente en relaciones abusivas y confundida con celos y cuidados. En las redes sociales, esto se puede fomentar a través de publicaciones, así como un espacio de apoyo e intercambio de experiencias. El objetivo fue analizar los comen-tarios publicados en las redes sociales relacionados con la violencia contra las mujeres y su asociación con la violencia psicológica. Se utilizó el método de la netnografía, mediante el análisis textual de los comentarios de un video publicado en la red social Facebook denominado: "Não confunda amor com abuso". Se transcribieron 571 comentarios de la publicación, siendo analizado el corpus por el software Iramuteq. Los comentarios analizados muestran que las cuestiones culturales contribuyen a la banalización de la violencia contra a mujer, especialmente la psicológica, ya que generalmente solo se considera violencia cuando hay agresión física. Uno se da cuenta de la importancia del trabajo psicoeducativo, por ejemplo, a través de videos de orientación, desti-nados a sensibilizar a la población y posible reducción de la violencia. (AU)

O ensino da Bioética nos cursos de Saúde Coletiva de universidades públicas brasileiras

Objetivo: Realizar um mapeamento das universidades públicas brasileiras que ofertam o curso de Saúde Coletiva, de modo a analisar a inserção de conteúdos relacionados à Bioética (disciplina e conteúdos curriculares). Método: Estudo de caráter descritivo, exploratório, com abordagem documental, realizado por meio do acesso aos projetos pedagógicos dos cursos de graduação em Saúde Coletiva, disponibilizados no portal Cadastro Nacional de Cursos e Instituições de Educação Superior, e nos sites oficiais das universidades. Resultados: Em abril de 2023, existiam 37 cursos de graduação em Saúde Coletiva no Brasil, sendo 17 deles, ofertados por universidades públicas. Ao analisar os projetos de curso, observou-se que ensino da Bioética ocorre de forma transversal e em diferentes componentes curriculares, com predomínio de conteúdos teóricos. Conclusão: O ensino da Bioética nos cursos de Saúde Coletiva pode ser aprimorado, inclusive com metodologias que favoreçam a problematização de situações cotidianas enfrentadas por sanitaristas brasileiros. Ademais, é necessário que as ações de ensino explorem diferentes perspectivas e princípios bioéticos, estimulando uma reflexão crítica, reflexiva e plural, sobre os desafios existentes.

Chemical composition of Piper gaudichaudianum Kunth essential oil and evaluation of its antimicrobial and modulatory effects on antibiotic resistance, antibiofilm, and cell dimorphism inhibitory activities.

Essential oils extracted from many plant species have different biological activities, among which microbial activity stands out. Species of the genus Piper have antimicrobial potential against different species of bacteria and fungi. In this sense, the present study aimed to determine the chemical composition of the essential oil from the leaves of Piper gaudichaudianum (EOPG), as well as to investigate their antimicrobial activity and their modulatory effect on the Norfloxacin resistance in the Staphylococcus aureus SA1199B strain overproducer of the NorA efflux pump. Furthermore, their inhibitory activities on the biofilm formation as well as on the cellular differentiation of C. albicans were evaluated. Gas chromatography analysis identified 24 compounds, such as hydrocarbon sesquiterpenes (54.8%) and oxygenated sesquiterpenes (28.5%). To investigate the antimicrobial potential of EOPG against S. aureus, E. coli, and C. albicans, a microdilution assay was performed, and no intrinsic antimicrobial activity was observed. On the other hand, the oil potentiated the activity of Norfloxacin against the SA1199B strain, indicating that EOPG could be used in association with Norfloxacin against S. aureus strains resistant to this antibiotic. EOPG also inhibited S. aureus biofilm formation, as evidenced by the crystal violet assay. In the dimorphism assay, EOPG was able to inhibit the cell differentiation process in C. albicans. Results indicate that EOPG could be used in association with Norfloxacin in the treatment of infections caused by resistant S. aureus strains overproducing the NorA efflux pump. Furthermore, its ability to inhibit the formation of hyphae by C. albicans suggests that EOPG could also be applied in the prevention and/or treatment of fungal infections.

Vasodilation promoted by (E,E)-farnesol involving ion channels in human umbilical arteries.

Background: (E,E)-farnesol is a sesquiterpene alcohol derived from plants and animals that exhibits pharmacological properties in the cardiovascular system. However, its effects on human umbilical vessels remain unknown. Purpose: Thus, this study aims to characterize the vasodilatory effect of (E,E)-farnesol in human umbilical arteries (HUA). Study design: The tissue is obtained from pregnant women over 18 years of age, normotensive, and without prepartum complications. After collected, the tissue was segmented and dissected to remove Wharton's jelly and obtain the umbilical arteries segments. Methods: HUA segments were isolated and sectioned into rings that were subjected to isometric tension recordings in an organ bath. Results: (E,E)-farnesol (1 µmol/L to 1 mmol/L) promoted vasodilatory effect in HUA preparations, affecting basal tone, and inhibiting the electromechanical coupling induced by KCl 60 mmol/L with greater potency (EC50 225.3 µmol/L) than the pharmacomechanical coupling induced by 5-HT 10 µmol/L (EC50 363.5 µmol/L). In the absence of extracellular calcium, pharmacomechanical coupling was also abolished, and contractions induced by CaCl2 or BaCl2 were attenuated by (E,E)-farnesol indicating a possible direct inhibition of L-type VOCC as a mechanism of the vasodilatory effect. The vasodilator efficacy of (E,E)-farnesol on reduction of vasocontraction induced by the presence of tetraethylammonium (1 or 10 mmol/L), 4-aminopyridine (1 mmol/L) and glibenclamide (10 µmol/L) suggesting a possible influence of different potassium channels (BKCa, KV and KATP). Conclusion: These results suggest that (E,E)-farnesol may be a promising pharmacological candidate for obstetric hypertensive disorders.

Transcultural Comparison of Mental Health and Work-Life Integration Blurring in the Brazilian and Spanish Populations during COVID-19.

The study aimed to compare the impact of Role Blurring on mental health and Work-Life Integration in the Brazilian and Spanish populations during COVID-19. Role Blurring, which is related to resources and demands in the work context, affects coping with stressors arising from role overlapping and impacts individuals' perception of work overload and mental health. The sample consisted of 877 adults from Spain (n = 498) and Brazil (n = 372), and various statistical analyses were conducted to compare the groups. Results showed that Role Blurring is linked to symptoms of anxiety, depression, and stress, as well as suicidal ideation. Therefore, it is essential to promote working conditions that limit expectations on availability and favor disconnection from work during leisure time. Public policies that intervene, promote, and prevent psychosocial risk factors in emergent contexts are crucial to prevent suicidal ideation and attempts. Considering the high expected influence of Blurring as a focus of interventions can be reflected in the medium term in the indicators of well-being and satisfaction of companies, institutions and organizations. This can result in the reduction of health costs aimed at cushioning post-COVID-19 impacts on mental health. The study is relevant to understand the impact of the pandemic and technology on mental health and suggests the need for interventions to promote work-life balance and prevent psychosocial risks.

Temporal assessment of entomological surveillance of Trypanosoma cruzi vectors in an endemic area of northeastern Brazil.

Entomological surveillance is essential for the control of triatomines and the prevention of Trypanosoma cruzi infection in humans and domestic animals. Thus, the objective of this study was to evaluate entomological indicators and triatomine control during the period from 2005 to 2015 in an endemic area in the state of Rio Grande do Norte, Brazil. This observational and retrospective study was developed based on data analysis related to active entomological surveillance activities and chemical control of infested housing units (HU) in the Agreste mesoregion of the state of Rio Grande do Norte, Brazil, in the period between 2005 to 2015. The quantitative analysis of housing units surveyed for entomological indicators was performed by linear regression of random effects (p < 0.05). The effect of the number of HU surveyed on the entomological indicators was analyzed by fitting a linear random effects regression model and an increasing intradomiciliary colonization rate was significant. In the period evaluated 92,156 housing units were investigated and the presence of triatomines was reported in 4,639 (5.0%). A total of 4,653 specimens of triatomines were captured and the species recorded were Triatoma pseudomaculata (n = 1,775), Triatoma brasiliensis (n = 1,569), Rhodnius nasutus (n = 741) and Panstrongylus lutzi (n = 568), with an index of natural infection by T. cruzi of 2.2%. Only 53.1% of the infested HU were subjected to chemical control. Moreover, there was a decrease in the total number of HU surveyed over time associated with an increase in the index of intradomiciliary colonization (p = 0.004). These data demonstrated that entomological surveillance and control of vectors in the Agreste mesoregion of the state has been discontinued, emphasizing the need for more effective public policies to effectively control the vectors, in order to avoid the exposure of humans and domestic animals to the risk of T. cruzi infection.

Musculoskeletal Injury Prevalence, Pain Perception, and Physical Activity Level Among Brazilian Strength and Cross-Training Practitioners.

In this study, we sought to determine the prevalence of musculoskeletal injuries, perceived pain, and physical activity level among Brazilian practitioners of strength training (ST) and functional fitness (FF). Participants were 311 men and women who trained in 10 FF training centers and seven ST gyms. Each participant completed surveys of the prevalence of musculoskeletal injuries, their pain perception, and their physical activity level. A chi square test was used to analyze associations between groups and distributions of injuries. When any significant difference was observed, the difference score was analyzed through the adjusted residual values. Fisher's exact test was used to determined the associations between musculoskeletal injury prevalence and training modality (FF and ST) and between musculoskeletal injury prevalence and practice frequency (times/wk). To measure the magnitude of association between variables, the Phi coefficient was calculated for 2x2 associations and Cramer's V was used whenever the distributions were outside this standard. When the dependent variable presented a dichotomous characteristic, an Odds Ratio (OR) was calculated with a confidence interval of 95%. We found a higher musculoskeletal injury prevalence in the axial skeleton (n = 52; 83.88%) in FF practitioners and in the lower limbs of ST practitioners (n = 9; 52.96%). When the physical activity level cutoff point was set at 300 minutes per week, there was a significant relationship between physical activity and training modality (p = 0.005). There was also a significant association between pain perception and musculoskeletal injury (p < 0.001). Clinical follow-up was a protective factor to being injured (OR = 0.18; CI = 0.06-0.49), and, even after multivariate analysis this significant association was maintained (OR = 0.03; CI = 0.01 - 0.08). Thus, FF practitioners reported more musculoskeletal injuries than STs, and follow-up medical or physical therapy was a protective factor to these injuries. FF practitioners also had a higher level of weekly physical activity weekly than ST practitioners. Functional fitness practitioners may be at a higher risk of injuries than those who participate in traditional strength training.